Amperometric glucose biosensor based on a surface treated nanoporous ZrO"2/Chitosan composite film as immobilization matrix [An article from: Analytica Chimica Acta] Review
Amperometric glucose biosensor based on a surface treated nanoporous ZrO"2/Chitosan composite film as immobilization matrix [An article from: Analytica Chimica Acta] Feature
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A surface treated nanoporous ZrO"2/Chitosan composite matrix was developed to fabricate the glucose biosensor. This material combined the advantages of inorganic nanoparticles, ZrO"2, and organic polymer, Chitosan. Glucose oxidase immobilized in the material maintained its activity well as the usage of glutaraldehyde was avoided. The activity of enzyme was 5.02 times greater than the cross-linked enzyme. The interaction between ZrO"2/Chitosan and enzyme was characterized with FT-IR spectroscopy. The results of transmission electron microscopy of surface-treated ZrO"2/Chitosan film showed that the matrix was porous and highly homogeneous. The parameters affecting the fabrication and experimental conditions of biosensors were optimized. The biosensor had a fast response of less than 10s. The linear range was 1.25 x 10^-^5 to 9.5 x 10^-^3M with a detection limit of 1.0 x 10^-^5M at 3@s and a sensitivity of 0.028@mAmM^-^1. The biosensor retained about 75.2% of its original response after one-month storage in a phosphate buffer.
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